Immunochemical study of protein profiles of Taleghan, Fars, and Lorestan strains of Mycoplasma agalactiae
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : R. Firouzi & M. H. Hosseini & Homa Orangi & M. Motamed & Gh. Moazeni
- چاپ و سال / کشور: 2010
Description
Agalactia is a contagious disease in sheep and goats. To prevent the disease, a vaccine containing three strains of Mycoplasma agalactiae namely Taleghan (T), Fars (F), and Lorestan (L) is currently used in Iran. In this study, the protein profiles of T, F, and L strains using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) with 8.5% gel and Western blotting were studied. Antigens were prepared from these strains, and the amounts of protein were measured using Lowry method9. Protein profiles of the three strains studied by SDS-PAGE revealed only minor differences with as many as 8, 14, and 10 bands detected in T, F, and L strains, respectively. Majority of the proteins were shared by all of the three strains. Western blotting with rabbit antisera against T, F, and L strains detected nine, nine, and eight immunodominant antigenic bands. All of the above strains revealed more or less similar protein profiles with major common immunodominant antigens at 117, 110, 98, 80, 50, and 5 KDa positions.
Comp Clin Pathol (2011) 20:39–42 DOI 10.1007/s00580-009-0946-0 Received: 15 September 2009 / Accepted: 25 November 2009 / Published online: 5 January 2010
