Genotype and antibiotic resistance profile of Escherichia coli strains involved in canine pyometra
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Reza Ghanbarpour & Baharak Akhtardanesh
- چاپ و سال / کشور: 2010
Description
Eighty-four Escherichia coli isolates from canine pyometra were examined to detect the antibiotic resistance profiles, phylogroup/subgroups and virulence genes. Twenty-seven (32.14%) antibiotic-resistant isolates were recovered, which were classified into 19 different antibiotic resistance patterns. The maximum resistance was against sulfadiazine and tetracycline. The antibiotic resistant isolates mostly belonged to A (40.74%) and D (29.62%) phylogroups. Among other examined isolates, 19.04% belonged to B1 phylogroup and remaining fell into six phylogenetic subgroups: A0 (25.00%), A1 (16.66%), B22 (10.77%), B23 (5.95%), D1 (14.28%), and D2 (8.33%). All isolates possessed at least one virulence gene and fimH was the most common gene. Frequency of other virulence genes were estimated as sfa/focD-E (58.33%), papE-F (44.04%), hlyA (34.52%), cnf1 (28.57%), iutA (17.85%), and afaIB-C and cdt genes (2.38%). Sixteen combinations of virulence genes were detected, with the combination cnf1, fimH, hlyA, papE-F, sfa/focD-E being the most prevalent pattern. None of the isolates contained the cnf2 and ipaH genes.
Comp Clin Pathol DOI 10.1007/s00580-010-1167-2 Received: 6 July 2010 / Accepted: 10 December 2010
