Use of the platelet function analyser PFA-100™ in juvenile pigs
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Constanze Leuser & Sibylle Schlottmann & Ralf Siekmann &Martin Heidt & Andreas Moritz & Natali Bauer
- چاپ و سال / کشور: 2010
Description
To evaluate the platelet function analyser PFA 100™ and impact of sample storage for pigs, samples from 24 German large white swine (3–5 months) were measured 15 and 30 min (n=24) and 1, 2, 4, 8 and 24 h (n=9) after sampling. For the PFA-100™ analyses, 10–20 ml sodium citrate anticoagulated blood were withdrawn from the vena femoralis using a 5-F catheter. K2EDTA anticoagulated blood (1.2 ml) was taken for haematological analysis. Using collagen/ADP cartridges, 10 of 93 (10.8%) analyses were invalid (9.7% aborted measurement, 1.1% organizational problems). With collagen/epinephrine cartridges, 28 of 93 (30.1%) measurements were invalid (22.6% aborted measurement, 6.45% closure time above the measurement range, 1.1% organizational problems). The median closure time at time point 15 min was 72.0 s (range, 50–122 s) for collagen/ADP and 123.5 s (range, 90–204 s) for collagen/ epinephrine. Invalid measurements were more frequent (P< 0.0001) with collagen/epinephrine cartridges. For both cartridges, no significant impact of storage time was seen (P=0.202 and 0.260). Mean haematocrit value was 0.27 l/ l (±0.04) and mean platelet count 343.5×109/l (±81.94). Therefore, collagen/epinephrine cartridges cannot be recommended to be used in pigs. In young animals with haematocrit values <0.25 l/l, prolonged closure times have to be considered, whereby the use of the PFA-100™ has to be seen critically. The wide reference range may limit its use to experimental settings rather than single individual measurements.
Comp Clin Pathol DOI 10.1007/s00580-010-1171-6 Received: 14 October 2010 / Accepted: 21 December 2010
