In vitro effects of Artemisia dracunculus essential oil on ruminal and abomasal smooth muscle in sheep
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ghader Jalilzadeh-Amin & Massoud Maham & Bahram Dalir-Naghadeh & Farshad Kheiri
- چاپ و سال / کشور: 2010
Description
Recently, essential oils have been substituted for synthetic antibiotics in improving rumen fermentation and animal performance. When ingested, along with their positive properties, some adverse effects may also be observed in animals. This study was primarily aimed at investigating the effects of the essential oils extracted from Artemisia dracunculus (EOAD) on ruminal and abomasal smooth muscle. EOAD was extracted using hydrodistillation. Sixteen compounds, representing 94.06% of the oil, were identified using gas chromatography/ mass spectrometry analyses, Hinokitiol (17.47%), estragole (17.28%), pulegone (10.23%), limonene (7.57%), methyl eugenol (7.46%), and bornyl acetate (7.12%) were the major compound in the oil. In the in vitro study, except for isolated ruminal strips, abomasal preparations from 24 healthy sheep, EOAD (0.1–100 μg/mL) evoked a weak spasmogenic effect followed by relaxation, but complete abolishment of spontaneous contraction occurred at the highest dose (1,000 μg/mL) (P<0.05). EOAD significantly (P<0.05) inhibited the Ach-induced contraction when tissues were pre-incubated with the highest doses. When animal rations are supplemented with essential oils, a reduced basal tone and an impaired response of the rumen and abomasal muscles to acetylcholine reflect hypotony and subsequently predispose the animals to abomasal displacement, abomasal rotation, and or indigestion.
Comp Clin Pathol DOI 10.1007/s00580-010-1155-6 Received: 18 July 2010 / Accepted: 7 December 2010
