Sphingosine-1-Phosphate Regulates the Expression of Adherens Junction Protein E-Cadherin and Enhances Intestinal Epithelial Cell Barrier Function
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Jose Greenspon Ruiyun Li Lan Xiao Jaladanki N. Rao Rex Sun Eric D. Strauch Terez Shea-Donohue Jian-Ying Wang Douglas J. Turner
- چاپ و سال / کشور: 2010
Description
Background The regulation of intestinal barrier permeability is important in the maintenance of normal intestinal physiology. Sphingosine-1-phosphate (S1P) has been shown to play a pivotal role in enhancing barrier function in several non-intestinal tissues. The current study determined whether S1P regulated function of the intestinal epithelial barrier by altering expression of E-cadherin, an important protein in adherens junctions. Methods Studies were performed upon cultured differentiated IECs (IEC-Cdx2L1 line) using standard techniques. Results S1P treatment significantly increased levels of E-cadherin protein and mRNA in intestinal epithelial cells (IECs) and also led to E-cadherin localizing strongly to the cell–cell border. S1P also improved the barrier function as indicated by a decrease in 14C-mannitol paracellular permeability and an increase in transepithelial electrical resistance (TEER) in vitro. Conclusions These results indicate that S1P increases levels of E-cadherin, both in cellular amounts and at the cell–cell junctions, and leads to improved barrier integrity in cultured intestinal epithelial cells.
Dig Dis Sci (2011) 56:1342–1353 DOI 10.1007/s10620-010-1421-0 Received: 14 January 2010 / Accepted: 2 September 2010 / Published online: 9 October 2010
