miR-137 Is Frequently Down-Regulated in Gastric Cancer and Is a Negative Regulator of Cdc42
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Qingjiang Chen Xiaobing Chen Mingzhi Zhang Qingxia Fan Suxia Luo Xinguang Cao
- چاپ و سال / کشور: 2011
Description
Introduction MicroRNAs (miRNAs) are a class of small (19–25 nucleotides) noncoding RNAs that regulate the expressions of a wide variety of genes, including some involved in cancer development. Some recent studies show that DNA methylation contributes to down-regulation of microRNA-137 (miR-137) during tumorigenesis. Whether down-regulation of miR-137 also exists in gastric cancer is unknown. Aim Our aim was to test the hypothesis that down-regulation of miR-137 also exists in gastric cancer. Methods Expression of levels of miR-137 were examined using real-time PCR on paired gastric cancer and adjacent non-cancerous tissues. The methylation status is detected by MSP. Results Results show that miR-137 is downregulated by hypermethylation of the promoter in gastric cancer tissues. Epigenetic silencing of miR-137 induced an up-regulation of its targets, Cdc42. Restoration of the miR-137 expression in gastric cancer cell lines downregulated the Cdc42 expression. Restoration of the miR-137 and inactivation of Cdc42 induce apoptosis and cell cycle G1 arrest in gastric cancer cells. Furthermore, the miR-137 expression was found to be inversely correlated with CDC42 expression in gastric caner. Conclusions miR-137 is frequently down-regulated in gastric cancer and is a negative regulator of Cdc42.
Dig Dis Sci (2011) 56:2009–2016 DOI 10.1007/s10620-010-1536-3 Received: 6 October 2010 / Accepted: 16 December 2010 / Published online: 8 January 2011
