Protective Effect of Rebamipide Against Helicobacter pylori-CagA-Induced Effects on Gastric Epithelial Cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Kyung Hwa Lee Jee-Yeon Kim Woo-Kyung Kim Dong-Hun Shin Kyung-Un Choi Dae Whan Kim Woo-Jeong Lee Jin-Hwa Choi Soo Han Lee Gwang Ha
- چاپ و سال / کشور: 2010
Description
Background Helicobacter pylori CagA dysregulates cell signaling pathways and leads to targeted transcriptional upregulation of genes implicated in gastric cell injury. The aim of this study was to determine the effects of rebamipide on CagA-induced effects on gastric epithelial cells. We investigated the effects of rebamipide treatment (pre- or post-treatment before or after CagA transfection) on CagA-induced gastric cell injury. Method We evaluated the morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization by confocal microscopy, IL-8 production by ELISA, and NF-jB activation by luciferase assay in AGS gastric epithelial cells and MDCK cells. Results Transfection of CagA into gastric epithelial cells induced morphologic changes (hummingbird phenotype), ZO-1 mislocalization, and IL-8 production in gastric epithelial cells. Pre-treatment with rebamipide inhibits CagA-induced effects on gastric epithelial cells, including morphologic changes (hummingbird phenotype) associated with ZO-1 mislocalization, IL-8 production, and NF-jB activity. Conclusions These results suggest that rebamipide might have a potential role in the protection of H. pylori CagAinduced effects on gastric epithelial cells.
Dig Dis Sci (2011) 56:441–448 DOI 10.1007/s10620-010-1299-x Received: 7 September 2009 / Accepted: 3 June 2010 / Published online: 16 June 2010
