Small Intestinal Bacterial Overgrowth in Nonalcoholic Steatohepatitis: Association with Toll-Like Receptor 4 Expression and Plasma Levels of Interleukin 8
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ahmed Abu Shanab Paul Scully Orla Crosbie Martin Buckley Liam O’Mahony Fergus Shanahan Sanaa Gazareen Eileen Murphy Eamonn M. M. Qui
- چاپ و سال / کشور: 2010
Description
Background Experimental and clinical studies suggest an association between small intestinal bacterial overgrowth (SIBO) and nonalcoholic steatohepatitis (NASH). Liver injury and fibrosis could be related to exposure to bacterial products of intestinal origin and, most notably, endotoxin, including lipopolysaccharide (LPS). Aim To compare the prevalence of SIBO and its relationships to LPS receptor levels and systemic cytokines in NASH patients and healthy control subjects. Methods Eighteen NASH patients (eight males) and 16 age-matched and gender-matched healthy volunteers were studied. SIBO was assessed by the lactulose breath hydrogen test (LHBT), plasma lipopolysaccharide binding protein (LBP) levels by ELISA, and expression (as a percentage) of TLR-2 and 4 on CD14-positive cells by flow cytometry. Pro-inflammatory cytokines (IL-1b, IL-6, IL-8, and TNF-a) were measured in plasma. Results SIBO was more common in NASH patients than control subjects (77.78% vs. 31.25%; P\0.0001). LBP levels and TLR-2 expression were similar in both groups, TLR-4/MD-2 expression on CD14 positive cells was higher among NASH patients: expression, mean ± SEM, NASH vs. control: 20.95 ± 2.91% vs. 12.73 ± 2.29%, P\0.05. Among the examined cytokines, only IL-8 levels were significantly higher in patients than control (P = 0.04) and correlated positively with TLR-4 expression (r = 0.5123, P = 0.036). Conclusion NASH patients have a higher prevalence of small intestinal bacterial overgrowth which is associated with enhanced expression of TLR-4 and release of IL-8. SIBO may have an important role in NASH through interactions with TLR-4 and induction of the pro-inflammatory cytokine, IL-8.
Dig Dis Sci (2011) 56:1524–1534 DOI 10.1007/s10620-010-1447-3 Received: 1 July 2010 / Accepted: 20 September 2010 / Published online: 3 November 2010
