Enhanced Expression of CXCL13 in Human Helicobacter pylori-Associated Gastritis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Yujiro Nakashima Hajime Isomoto Kayoko Matsushima Akira Yoshida Toshiyuki Nakayama Masaaki Nakayama Junzo Hisatsune Tatsuki Ichikawa F
- چاپ و سال / کشور: 2011
Description
Background and Aims Chemokine CXC ligand 13 (CXCL13) and CXC receptor type 5 (CXCR5) are constitutively expressed in tertiary lymphoid follicles where the CXCL13/CXCR5 system regulates B lymphocytes homing. In this study, we sought to examine CXCL13 expression in the H. pylori-infected and -uninfected gastric mucosa and to elucidate the implication in the pathogenesis of HAG in humans. Methods Using endoscopic biopsies taken from the gastric antrum of 29 subjects infected with Helicobacter pylori and 22 uninfected subjects, mucosal CXCL13 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results The CXCL13 expression levels were significantly more elevated in H. pylori-positive patients than uninfected ones. The CXCL13 expression levels correlated with the degree of chronic gastritis and bacterial colonization. Immunohistochemistry and in vitro infection assay showed that CXCL13 was not produced by the gastric epithelium, but the a-smooth muscle antigen expressing mesenchymal cells were the possible source of CXCL13 within H. pyloriinfected gastric mucosa. CXCR5 immunostaining was seen in the CD20-positive lymphoid aggregates. Conclusions The enhanced induction of CXCL13 may be involved in the pathogenesis of H. pylori-associated gastritis
Dig Dis Sci DOI 10.1007/s10620-011-1717-8 Received: 6 December 2010 / Accepted: 8 April 2011
