Apoptotic cell clearance in chronic inflammation of lateral neck cysts
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Wieslaw Dobros Karolina Burda Krzysztof Guzik Joanna Koziel Jan Potempa
- چاپ و سال / کشور: 2011
Description
The mechanism driving accumulation of large numbers of apoptotic and necrotic neutrophils in inflamed lateral neck cysts (LNC), in the absence of infection, remains obscure. The cellular content of cysts obtained from 17 patients was co-cultured with human macrophages. Phagocytosis levels of cyst-derived neutrophils were determined and compared to the uptake of spontaneously apoptotic neutrophils. Simultaneously, the expression of cytokines in macrophages exposed to cyst contents was measured. In comparison to spontaneously apoptotic neutrophils, the phagocytosis of LNC-derived neutrophils by macrophages was inefficient. An inverse correlation between neutrophil content in LNC and their uptake was observed. Macrophages co-cultured with cyst contents responded with variable expression of IL-6, TNFa and IL-10. The hindered clearance of apoptotic neutrophils in LNC may lead to secondary necrosis of these cells and stimulation of the inflammatory reaction. Together with local production of anti-inflammatory cytokines, this may fuel chronic inflammation in the cysts.
Eur Arch Otorhinolaryngol DOI 10.1007/s00405-011-1703-y, Received: 30 March 2011 / Accepted: 29 June 2011
