Optimization of simultaneous analysis of tetrodotoxin, 4-epitetrodotoxin, 4,9-anhydrotetrodotoxin, and 5,6,11-trideoxytetrodotoxin by hydrophilic interaction liquid chromatography–tandem mass spectrometry
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Mari Yotsu-Yamashita Jun-Ho Jang Yuko Cho Keiichi Konoki
- چاپ و سال / کشور: 2010
Description
Recently, liquid chromatography–mass spectrometry (LC–MS) has been recognized as a useful method to analyze tetrodotoxin (TTX), the primary toxin for puffer fish poisoning. TTX usually exists with its chemically interchangeable analogs, such as 4-epiTTX and 4, 9-anhydroTTX. TTX and 4-epiTTX have the same molecular weight and the same fragmentation pattern by tandem mass spectrometry (MS–MS). In this study, we optimized conditions for hydrophilic interaction liquid chromatography (HILC)–MS–MS to quantitate TTX, 4-epiTTX, 4,9-anhydroTTX, and 5,6,11-trideoxyTTX with good separation. The relationships between the applied amounts of each TTX analog to the HILC–MS–MS instrument and the peak areas showed good linearity in the ranges examined. The lower limits of detection (signal-to-noise ratio = 3) were 64 pg on-column for TTX, 128 pg for both 4-epiTTX and 4,9-anhydroTTX, and 180 pg for 5,6,11-trideoxyTTX using an API 2000 mass spectrometer.
Forensic Toxicol (2011) 29:61–64 DOI 10.1007/s11419-010-0106-x] Received: 6 August 2010 / Accepted: 18 November 2010 / Published online: 21 December 2010
