Determination of acephate and methamidophos in tissues: appearance of matrix effect in gas chromatography–mass spectrometry
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Nobuyuki Adachi Hiroshi Kinoshita Minori Nishiguchi Motonori Takahashi Harumi Ouchi Takako Minami Kiyoshi Matsui Takehiko Yamamura Shi
- چاپ و سال / کشور: 2011
Description
A simple and reliable method to determine acephate and methamidophos in mammalian tissues is presented. The method includes solid-phase extraction of tissue extracts with active carbon cartridges followed by gas chromatography–mass spectrometry analysis. During the study, a matrix effect was observed especially at low concentrations of acephate and methamidophos in serum and in brain. To minimize the effect, we prepared calibration curves with relatively short ranges. The validation data, such as the linearity of calibration curves, limits of detection, and coefficients of variation for recovery rates, were generally satisfactory. The present method is useful for determination of acephate and methamidophos in mammalian tissues because of its simplicity and speed, keeping in mind the presence of the matrix effect.
Forensic Toxicol (2011) 29:159–162 DOI 10.1007/s11419-011-0110-9 Received: 1 February 2011 / Accepted: 4 March 2011 / Published online: 29 March 2011
