The effect of doxycycline temperature-sensitive hydrogel on inhibiting the corneal neovascularization induced by BFGF in rats
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Wenru Su & Zhanrong Li & Miaoli Lin & Yongping Li & Zixin He & Chuanbin Wu & Dan Liang
- چاپ و سال / کشور: 2010
Description
Background To study the effect of doxycycline temperaturesensitive hydrogel (DTSH) on inhibiting the corneal neovascularization (NV) induced by the basic fibroblast growth factor (bFGF). Methods Corneal NV was induced by slow-release polymer pellets containing bFGF, using a rat corneal pocket model. After being implanted with bFGF pellets, the female Sprague–Dawley rats were randomly divided into seven groups (12 rats/group). The grouped rats were given topically normal saline solution and neutralized DTSH at a concentration of 0%, 0.01%, 0.05%, 0.1%, 0.5%, and 1% respectively, and treated for 6 consecutive days. After 6 days of treatment, the cornea was perfused with India ink. The length and area of the corneal vessel were measured and analyzed by Image Pro-Plus 5.1. Results Compared to the control group given saline solution, the study groups given DTSH at a concentration of 0.05%, 0.1%, 0.5%, and 1% showed significant reduction in the vessel length (respectively, 58%, 60%, 52%, and 37%) and the vessel area (respectively, 61%, 62%, 49%, and 39%) (p<0.001). However, no such significant reduction was observed in the study group given 0.01% DTSH (p=0.133 and 0.166 for vessel length and area respectively). Study groups given 0.05% and 0.1% DTSH showed better effects than groups given 0.01% and 1% DTSH with regard to reducing the vessel length and the vessel area (p<0.05). Conclusion The study results showed that topical DTSH effectively inhibited corneal NV at the ideal concentration of 0.05% and 0.1%. Therefore, topical DTSH could be considered as an alternative treatment for the clinical management of corneal NV.
Graefes Arch Clin Exp Ophthalmol (2011) 249:421–427 DOI 10.1007/s00417-010-1539-y Received: 1 May 2010 / Revised: 23 September 2010 / Accepted: 27 September 2010 / Published online: 16 October 2010
