Cardiac and vascular gene profiles in an animal model of takotsubo cardiomyopath
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Takashi Ueyama Yuta Yamamoto Kazuki Ueda Tetsuya Kawabe Takuzo Hano Takao Ito Yoshihiro Tsuruo Masao Ichinose Ken-ichi Yoshid
- چاپ و سال / کشور: 2010
Description
We investigated cardiac and vascular gene profiles in response to immobilization stress (IMO) in rats, an animal model of emotional stress-induced takotsubo cardiomyopathy using microarray analysis, followed by re-confirmation with real-time reverse transcription-polymerase chain reaction. Expression levels of the identified genes were further estimated by pretreatment with an a1-adrenoceptor blocker and/or a b1-adrenoceptor blocker. In response to IMO, expression of 46 genes was significantly altered in the heart and that of 49 genes was significantly altered in the aorta. Pathway analysis with DAVID Bioinformatics Resources indicated that regulation of transcription and response to endogenous stimulation were the top two scoring pathways. Altered expression of cardiac genes was blunted by pretreatment with a b1-adrenoceptor blocker or a1 ? b1-adrenoceptor blockers. In contrast, that of aortic genes was blunted by pretreatment with an a1-adrenoceptor blocker or a1 ? b1-adrenoceptor blockers. Activation of a1-adrenoceptor in the blood vessels or activation of b1-adrenoceptors in the heart were mainly responsible for emotional stress-induced alteration of cardiac and vascular gene profiles.
Heart Vessels (2011) 26:321–337 DOI 10.1007/s00380-010-0070-8 Received: 5 January 2010 / Accepted: 9 July 2010 / Published online: 3 December 2010
