Abundant expression of KCNE1 in the left ventricle of the miniature pig
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Kaori Soma Kentaro Nagaoka Masayoshi Kuwahara Hirokazu Tsubone Koichi Ito
- چاپ و سال / کشور: 2010
Description
Delayed rectifier potassium currents such as IKr and IKs play an important role in the repolarization phase of the action potential in cardiac myocytes. Electrophysiological studies have shown that the pig is a useful animal not only for clinical use as a good candidate for humans, but also for basic research in heart function or arrhythmia. However, no studies concerning the potassium channels on a molecular level have been done. To elucidate the expression level and distribution of delayed rectifier potassium channels in pigs, we quantitatively investigated the IKr and IKs channel subunits using the real-time polymerase chain reaction (PCR) method. The hearts from Clawn miniature pigs were separated into the apical and basal regions, and subsequently excised into transmural trisections within each of the left ventricular walls, epicardium, midcardium, and endocardium. After RNA extraction from these sites, real-time PCR was executed with reverse transcriptional products for quantitative analysis. The expression level of KCNE1 was significantly higher than those of KCNQ1, KCNH2, and KCNE2, which were comparable in all sites. Transmural heterogeneity of these potassium channel subunits was not detected on the mRNA level. These results indicate that KCNE1 is a dominant su
Heart Vessels (2011) 26:353–356 DOI 10.1007/s00380-010-0041-0 Received: 1 September 2009 / Accepted: 18 March 2010 / Published online: 27 October 2010
