Scanty integration of osteochondral allografts cryopreserved at low temperatures with dimethyl sulfoxide
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Francisco Forriol • Umile Giuseppe Longo • Eduardo Alvarez • Stefano Campi • Purificacion Ripalda • Carla Rabitti • Nicola Maffulli • Vincenzo Denaro
- چاپ و سال / کشور: 2010
Description
Purpose To compare the integration of osteochondral allografts cryopreserved at different temperatures and different concentrations of dimethyl sulfoxide in an in vivo sheep animal model. Methods Thirty-six adult sheep were randomly allocated to 6 groups of allograft osteochondral transplantation. Six osteochondral cylinders were stored for 6 weeks at -80C; 6 at -80C with 10% dimethyl sulfoxide (DMSO); 6 at -80C with 10% DMSO for 90 min; 6 at -186C; 6 at -186C with 10% DMSO; 6 at -186C for 90 min. After transplantation, all animals were euthanized at 6 months. Harvested specimens underwent gross morphologic and histologic evaluation. Results We found no statistically significant differences when comparing the gross cartilage morphology and histopathologic scores of each group. The Mankin and OARSI scores and the modified Wakitani and OARSI scores showed a good correlation grade. The Mankin and modified Wakitani scores showed a fair correlation grade. Conclusion The cryopreservation protocols adopted in the present study provided scanty integration in an in vivo sheep model of osteochondral allograft transplantation. Therefore, their use in the clinical practice is discouraged
Knee Surg Sports Traumatol Arthrosc (2011) 19:1184–1191 DOI 10.1007/s00167-010-1317-8 Received: 21 November 2009 / Accepted: 21 October 2010 / Published online: 6 November 2010
