Effects of low-level laser therapy on expression of TNF-ل and TGF-â in skeletal muscle during the repair process
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Raquel Agnelli Mesquita-Ferrari &Manoela Domingues Martins & Jose Antônio Silva Jr. & Tatiana Dias da Silva & Roberto Farina Piovesan & Vanessa Chri
- چاپ و سال / کشور: 2011
Description
The aim of the present study was to determine the effect of low-level laser therapy (LLLT) on the expression of TNF-ل and TGF-â in the tibialis anterior muscle of rats following cryoinjury. Muscle regeneration involves cell proliferation, migration and differentiation and is regulated by growth factors and cytokines. A growing body of evidence suggests that LLLT promotes skeletal muscle regeneration by reducing the duration of acute inflammation and accelerating tissue repair. Adult male Wistar rats (n=35) were randomly divided into three groups: control group (no lesion, untreated, n=5), cryoinjury without LLLT group (n=15), and cryoinjury with LLLT group (n=15). The injured region was irradiated three times a week using an AlGaInP laser (660 nm; beam spot 0.04 cm2, output power 20 mW, power density 500 mW/cm2, energy density 5 J/cm2, exposure time 10 s). Muscle remodeling was evaluated at 1, 7 and 14 days (long-term) following injury. The muscles were removed and total RNA was isolated using TRIzol reagent and cDNA synthesis. Real-time polymerase chain reactions were performed using TNF-ل and TGF-â primers; GAPDH was used to normalize the data. LLLT caused a decrease in TNF-ل mRNA expression at 1 and 7 days following injury and in TGF-â mRNA expression at 7 days following cryoinjury in comparison to the control group. LLLT modulated cytokine expression during short-term muscle remodeling, inducing a decrease in TNF-ل and TGF-â.
Lasers Med Sci (2011) 26:335–340 DOI 10.1007/s10103-010-0850-5 Received: 14 June 2010 / Accepted: 7 October 2010 / Published online: 4 November 2010
