Ultra-high field diffusion tensor imaging of articular cartilage correlated with histology and scanning electron microscop
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : José G. Raya · Andreas P. Arnoldi · Daniel L. Weber · Lucianna Filidoro · Olaf Dietrich · Silvia Adam-Neumair · Elisabeth Mützel · Gerd Melkus · Rei
- چاپ و سال / کشور: 2011
Description
Object To investigate the relationship of the different diffusion tensor imaging (DTI) parameters (ADC, FA, and first eigenvector (EV)) to the constituents (proteoglycans and collagen), the zonal arrangement of the collagen network, and mechanical loading of articular cartilage. Material and methods DTI of eight cartilage-on-bone samples of healthy human patellar cartilage was performed at 17.6 T. Three sampleswere additionally imaged under indentation loading. AfterDTI, samples underwent biomechanical testing, safranin-O staining for semiquantitative proteogly- can estimation, and scanning electronmicroscopy (SEM) for depicting collagen architecture. Results From the articular surface to the bone–cartilage interface, ADC continuously decreased and FA increased. Cartilage zonal heights calculated from EVs strongly correlated with SEM-derived zonal heights (P <0.01, r 2=0.87). Compression reducedADCin the superficial30%of cartilage and increased FA in the superficial 5% of cartilage. Reorientation of the EVs indicative of collagen fiber reorientation under the indenter was observed. No significant correlation was found between ADC, FA, and compressive stiffness. Conclusions Correlating ADC and FA with proteoglycan and collagen content suggests that diffusion is dominated by different depth-dependent mechanisms within cartilage. Knowledge of the spatial distribution of the DTI parameters and their variation contributes to form a database for future analysis of defective cartilage.
Magn Reson Mater Phy (2011) 24:247–258 DOI 10.1007/s10334-011-0259-6 Received: 5 January 2011 / Revised: 26 April 2011 / Accepted: 5 May 2011 / Published online: 1 June 2011
