REG gamma: a potential marker in breast cancer and effect on cell cycle and proliferation of breast cancer cell
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Xiaoyi Wang • Shengfen Tu • Jinxiang Tan • Tian Tian • Liang Ran • Jean-Francois Rodier • Guosheng Ren
- چاپ و سال / کشور: 2010
Description
To investigate the expression and significance of proteasomes reactivator REG gamma (c) in breast cancer. First, we showed the expression of REGc in breast cancer, metastatic lymph nodes and normal breast tissues. Meanwhile, we also analyzed the relationship between REGc and estrogen receptor (ER), CerBb-2, lymph nodes metastasis and clinical stage of breast cancer. REGc expression was determined by immunohistochemical staining and western blot. Secondly, we detected the expression of REGc and REGc-mRNA in human breast cancer cell lines (MDA-MB-231, MCF-7) and human breast ductal epithelial cell line (HBL-100) by western blot and real-time PCR. Finally, in order to identify effect of REGc on breast cancer cell cycle and proliferation, we constructed recombinant plasmid of PcDNA3.1-REGc and designed siRNA for REGc in vitro. Cell cycle was assayed by flow cytometer (FCM), proliferation was measured by methyl thiazolyl tetrazolium (MTT). The results demonstrated abnormal high expression of REGc in breast cancer and its metastatic lymph nodes. REGc expression was related to breast cancer and its status of ER, CerBb-2 and lymph nodes metastasis. REGc is one of the potential markers in breast cancer. REGc could facilitate the growth of breast cancer cells.
Med Oncol (2011) 28:31–41 DOI 10.1007/s12032-010-9546-8 Received: 7 June 2009 / Accepted: 11 September 2009 / Published online: 14 May 2010
