Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Shi-Lian Hu • Da-Bing Huang • Yu-Bei Sun • Lei Wu • Wei-Ping Xu • Shi Yin • Jiong Chen • Xiao-Dong Jiang • Gan Shen
- چاپ و سال / کشور: 2010
Description
Runx3 and CHFR genes were defined as tumor suppressor genes in gastric cancer (GC) recently. This paper was to investigate the roles of methylation and expression status of Runx3 and CHFR genes in GC patients. Methylation- specific polymerase chain reaction (MSP) and bisulfite DNA sequencing (BSP) were used to detect methylation status of Runx3 and CHFR genes in GC patients. The expression of Runx3 and CHFR in GC patients was analyzed by reverse transcription polymerase chain reaction (RT– PCR) and immunohistochemical analysis. The expression of the protein and mRNA decreased remarkably in the patients with aberrant promoter methylation of Runx3 and CHFR genes. The methylation status of Runx3 and CHFR were inversely related to the tumor size, tumor invasion depth and tumor differentiation in GC patients. Moreover, the protein expression of Runx3 andCHFR were significantly correlated with tumor invasion depth and tumor differentiation, respectively. Aberrant promoter methylation of Runx3 and CHFR genes may be involved in the carcinogenesis and development of GC and may provide useful clues for the prediction of the malignant behaviors of GC
Med Oncol (2011) 28:447–454 DOI 10.1007/s12032-010-9467-6 Received: 30 January 2010 / Accepted: 24 February 2010 / Published online: 19 March 2010
