Ischemic preconditioning attenuates of ischemia-induced degradation of spectrin and tau: implications for ischemic tolerance
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Takayuki Nakajima • Syoichi Ochi • Chika Oda • Maki Ishii • Kazushige Ogawa
- چاپ و سال / کشور: 2010
Description
Global ischemia selectively induces CA1 neuronal death in the hippocampus. Pretreatment with nonlethal ischemia (i.e. ischemic preconditioning) prevents CA1 neuronal death induced by lethal ischemia. While ischemic tolerance is a well-known phenomenon, the underlying molecular mechanisms are not fully understood. Cytoskeletal proteins including a-spectrin, tau, and microtubule-associated protein 2 (MAP-2) are indispensable for the maintenance of neuronal homeostasis. Here, we report the effects of ischemic preconditioning on the ischemia-induced degradation of cytoskeletal proteins a-spectrin, tau, and MAP-2 in the rat CA1 region. We found that most neurons of the CA1 region had died after 5 min of ischemia. However, exposing the brain to 3 min of ischemic preconditioning 3 days earlier significantly reduced the number of neuronal death. A significant degradation of a-spectrin and tau, but not of MAP-2, was found in the CA1 region after 5 min of ischemia. Ischemic preconditioning attenuated the ischemia-induced massive degradation of a-spectrin and tau. Our results suggest that the attenuation of ischemia-induced degradation of a-spectrin and tau by ischemic preconditioning may be associated with the neuroprotective mechanism of the ischemic tolerance.
Neurol Sci (2011) 32:229–239 DOI 10.1007/s10072-010-0359-5 Received: 7 August 2009 / Accepted: 15 June 2010 / Published online: 2 July 2010
