Therapeutic time window for the neuroprotective effects of NGF when administered after focal cerebral ischemia
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ji-Ping Yang • Huai-Jun Liu • Hua Yang • Ping-Yong Feng
- چاپ و سال / کشور: 2011
Description
In the present study, we evaluated the neuroprotection time window for nerve growth factor (NGF) after ischemia/reperfusion brain injury in rabbits as related to this anti-apoptosis mechanism. Male New Zealand rabbits were subjected to 2 h of middle cerebral artery occlusion (MCAO), followed by 70 h of reperfusion. NGF was administered after injury to evaluate the time window. Neurological deficits, infarct volume, neural cell apoptosis and expressions of caspase-3 and Bcl-2 were measured. Compared to saline-treated control, NGF treatment at 2, 3 and 5 h after MCAO significantly reduced infarct volume, neural cell apoptosis and expression of caspase-3 (P\0.01), up-regulated the expression of Bcl-2 and improved functional recovery (P\0.01). However, treatment at latter time points did not produce significant neuroprotection. Neuroprotection treatment with NGF provides an extended time window of up to 5 h after ischemia/reperfusion brain injury, in part by attenuating the apoptosis.
Neurol Sci (2011) 32:433–441 DOI 10.1007/s10072-011-0512-9 Received: 6 December 2009 / Accepted: 24 February 2011 / Published online: 16 March 2011
