Quantitative Measurement of Serum Hepatitis B Surface Antigen Using an Immunoradiometric Assay in Chronic Hepatitis B
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Hyun-Woo Kwon & Ho-Young Lee & Seog Gyun Kim & Won Kim & Yong Jin Jung & Keon Wook Kang & June-Key Chung & Myung Chul Lee & Dong Soo Lee
- چاپ و سال / کشور: 2010
Description
Purpose Measurement of serum hepatitis B virus surface antigen (HBsAg) levels is important for the management of chronic hepatitis B patients in terms of monitoring response to antiviral therapy. This study aimed to evaluate the diagnostic performance of a new diagnostic kit, which quantitatively measures serum HBsAg level using an immunoradiometric assay (IRMA)-based method. Measurements were compared with those obtained using a chemiluminescent microparticle immunoassay (CMIA)-based method. Methods The blood samples of 96 patients with chronic hepatitis B were used in this study. Copy numbers of serum hepatitis B virus (HBV) DNA were determined in 23 of these samples. The correlation between and the concordance of IRMA and CMIA results were determined using Pearson’s correlation coefficients. P values of 0.05 were considered to be statistically significant throughout. Results Laboratory diagnoses based on IRMA were wholly in agreement with those based on CMIA. Furthermore, serum HBsAg levels by IRMA were found to be highly correlated with those determined by CMIA (correlation coefficient R2 = 0.838, p<0.001). Serum HBsAg level and serum HBV DNA copies were found to be linearly related by both methods (R2 = 0.067, p=0.316 by IRMA, and R2 = 0.101, p=0.215 by CMIA). Conclusion The diagnostic performance of the investigated IRMA method of determining HBsAg levels was found to be comparable with that of a CMIA-based method in chronic hepatitis B patients
Nucl Med Mol Imaging (2011) 45:15–20 DOI 10.1007/s13139-010-0061-9 Received: 15 September 2010 / Accepted: 18 October 2010 / Published online: 16 November 2010
