Dental caries experience in relation to salivary findings and molecular identification of S. mutans and S. sobrinus in subjects with Down syndrome
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Aline Roge´ria Freire de Castilho • Vanessa Pardi • Ca´ssio Vicente Pereira
- چاپ و سال / کشور: 2011
Description
This study investigated the association between clinical and salivary or molecular parameters in Down syndrome subjects. Sixty individuals (1- to 48-year old) were clinically examined using DMFT/DMFS. Stimulated saliva was collected; salivary flow was calculated (mL/ min), buffering capacity was measured using a standard pH tape. In addition, 25 lL of saliva was diluted using 10-fold-dilution method and then placed on Mitis-Salivarius- Bacitracin agar to count colony forming units (CFU/ mL) of mutans streptococci. Polymerase chain reaction analysis identified species. Caries indexes were 0.65–13.5 (DMFT) and 0.65–26.0 (DMFS) according to groups. Ninety-four percent of subjects had low flow rate (0.7–1.0 mL/min) and 44% had low buffering capacity (pH\4). Besides, 60% had more than 1 9 106 CFU/mL, 60% had S. mutans, and 41.4% had S. sobrinus. Caries indexes did not significantly correlate with flow rate, buffering capacity, CFU/mL by Pearson’s correlation (p[0.05), and showed no significant association with prevalence of species by Chi-square (p[0.05). There is no association between clinical picture and salivary or molecular parameters in Down syndrome subjects.
Odontology DOI 10.1007/s10266-011-0010-9 Received: 25 June 2010 / Accepted: 26 November 2010
