Expression and function of the Ets transcription factor pea3 during formation of zebrafish pronephros
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Qiuxia Chen & Songming Huang & Qingshun Zhao & Ronghua Chen & Aihua Zhang
- چاپ و سال / کشور: 2011
Description
Polyomavirus enhancer activator 3 (Pea3), belonging to the PEA3 subfamily of Ets transcription factors, is essential for certain organogenesis in mammals. Previously, we found that pea3 correlated with wt1 expression and may contribute to nephrogenesis in rats. Here, we observed that pea3 was mainly expressed in the zebrafish pronephric glomerulus. We further performed functional analyses by in situ hybridization of pea3 in zebrafish embryos after pea3 messenger RNA (mRNA) overexpression and inhibition of double-target genes (pea3 and erm, another member of the PEA3 subfamily) by antisense morpholino-oligonucleotides (MO). Overexpression of pea3 induced abnormal pronephrogenesis. However, MO-pea3 coinjected with MO-erm, but not alone, inhibited zebrafish pronephros development, and these defects were rescued by overexpression of the zebrafish wt1a gene. Thus, pea3 and erm are required for zebrafish pronephrogenesis and can functionally complement each other, and the wt1a gene may be one of their downstream targets.
Pediatr Nephrol (2011) 26:391–400 DOI 10.1007/s00467-010-1713-9 Received: 15 March 2010 / Revised: 23 October 2010 / Accepted: 25 October 2010 / Published online: 24 December 2010
