In vitro cytokine production by mononuclear cells exposed to bone cement extracts
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Donatella Granchi!,*, Gabriela Ciapetti!, Federica Filippini!, Susanna Stea!, Elisabetta Cenni!, Arturo Pizzoferrato!, Aldo Toni"
- چاپ و سال / کشور: 2000
Description
The authors evaluated the ability of bone cement to modify the pro"le of pro-in#ammatory cytokines secreted by the immune cells. Peripheral blood mononuclear cells (PBMC) collected from healthy individuals were cultured with cement extracts and tested to assess the release of IL-1b, TNFa, GM-CSF and IL-6 in both unstimulated and PHA-stimulated PBMC. The cytokine release of unstimulated PBMC was very poor, and in particular the IL-1b was undetectable: the addition of cement extract increased both TNFa and GM-CSF release and decreased IL-6, sometimes signi"cantly. The most recurrent observation in PHA-stimulated PBMCs exposed to bone cement extract was the increase in both IL-1b and IL-6 release, while both the mean concentration and the index of release of TNFa and GM-CSF were changeable. In conclusion our results showed that leachable components of some bone cements can induce in vitro the release of pro-in#ammatory cytokines which are known to be involved in the bone resorption associated with aseptic loosening of hip prostheses. These "ndings allowed us to identify materials endowed with the highest in#ammatory power. ( 2000 Elsevier Science Ltd. All rights reserved.
Biomaterials 21 (2000) 1789}1795 Received 11 August 1999; accepted 16 January 2000
