Cytokine release in mononuclear cells of patients with Co-Cr hip prosthesis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Donatella Granchi!,*, Gabriela Ciapetti!, Susanna Stea!, Lucia Savarino!, Federica Filippini!, Alessandra Sudanese", Gianfranco Zinghi#, Lucio Montana
- چاپ و سال / کشور: 1999
Description
The aim of this study was the evaluation of the release of bone-resorbing cytokines in peripheral blood mononuclear cells (PBMC) of patients with aseptic loosening of Co}Cr hip prostheses. TNF-a, IL-6, and GM-CSF were measured in both unstimulated and PHA-stimulated PBMC, and in PBMC cultured in the presence of chromium and cobalt extracts. Blood samples from healthy donors were used as controls. Serum samples of both patients and healthy donors were tested to determine the concentration of metal ions. The proportion of lymphocyte, monocyte and lympocyte subpopulation in cultured PBMC did not di!er in patients and the control group. In unstimulated PBMC the release of TNF was signi"cantly higher in patients than in the control group, while IL-6 was signi"cantly decreased and no change was observed for GM-CSF. When the PBMC were challenged with chromium extract, all the &index of cytokine release' resulted higher in patients than in the control group; cobalt extract increased both the TNF and GM-CSF index, but not the index of IL-6 release. Metal concentrations in serum from patients were signi"cantly increased and correlated with the TNF release in PBMC stimulated with both metal extract. Our results suggest that a CoCr-implant releases a large amount of metal ions which could mediate the priming or the renewal of a cell-mediated hypersensitivity reaction. The prevalence of circulating lymphocytes responsible for the delayed hypersensitivity, namely Th1, would justify both the signi"cant increase of TNF and the signi"cant decrease of IL6 in unstimulated PBMC of patients, as well as the signi"cant increase of the &index of cytokine release' after the challenge with metal ions. ( 1999 Elsevier Science Ltd. All rights reserved
Biomaterials 20 (1999) 1079-1086 Received 22 July 1998; accepted 17 December 1998
