Thermally controlled protein release from gelatin±dextran hydrogels
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Y. Aso*, S. Yoshioka, Y. Nakai, S. Kojima
- چاپ و سال / کشور: 1999
Description
Biodegradable hydrogels in which drug release was controlled by sol-gel transition were prepared. Gelatin was used as a component because it exhibits sol-gel transition in response to temperature changes. Glycidyl methacrylated (GMA) dextran was crosslinked by low dose g-irradiation in the presence of gelatin and the model drugs, b-galactosidase (b-GA), bovine serum albumin (BSA) or 5-پPuorouracil (5-FU). The enzyme activity of b-GA remained greater than 95% after irradiation. Temperatureپ}responsive release of b-GA and BSA resulted from the sol-gel transition of gelatin. Sol-gel transition was conRrmed by the temperature dependence of the spinپ}spin relaxation time of the gel polymer protons. The protein release rate was a€ected by both the degree of GMA substitution and the gelatin concentration. Desired release rate could be achieved by adjusting these factors. The release rate of 5-FU was not a€ected by the sol-gel transition of gelatin.
Radiation Physics and Chemistry 55 (1999) 179-183 Received 20 July 1997; accepted 10 April 1998
