Effect of additives and fed-batch culture strategies on the production of glutathione by recombinant Escherichia coli
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Yin Li'* Jian Chen, a Ying-ying Mao, Shi-yi Lun ' and Yoon-mo KOO b
- چاپ و سال / کشور: 1998
Description
The effect of additives inclucling yeast extract, precursor amino acids and ATP on the production of glutathione (GSH) by recombinant E. coil was investigated. The addition of precursor amino acids (glutamate, cysteine and glycine) and ATP improved intracellular GSH accumulation. When 2.0 g/l ATP and 9 mmol/l precursor amino acids were added at the beginning and after 12 h of fermentation, respectively, cell concentration and intracellular GSH content was 24% and 1.4 times higher than that of culture without additions. Growth inhibition was observed while the initial glucose concentration was above 21) ~/1. Based on the analysis of the batch culture process, different fed-batch cultures in jar fermenters were carried out in which three kind of simple feeding strategies were applied. An exponential feeding strategy increased cell density, productivity and biomass yield. Using this strategy, cell density and total GSH quantity in broth reached 80 g(DCW)/I and 880 mg/l at 25 h without pure oxygen, respectively.
Process Biochemistry Vol. 33, No. 7, pp. 7119-714, 1998 Received 29 January 1998; revised version received q Februan' 1998; accepted 28 Fchrua~ 1998
