Modeling the protein partitioni ...
Aqueous polymer two-phase system provides a powerful method for separation and puri:cation of biomaterials. Among various factors, polymer concentrat ...
C omplex formation of a calcium ...
The elution of FLAG-fusions (an octapeptide with the sequence DYKDDDDK) from immobilized anti-FLAG antibody M1 cannot be explained by a switch of the ...
Polymer thermodynamics of adsor ...
Since proteins are polymers, their adsorption at interfaces should share some common features with polymers whose adsorption behavior is being rather ...
Protein–polysaccharide interact ...
If the most important parameters affecting protein–polysaccharide interactions are now well documented, recent advances concern the structure-buildin ...
The accuracy and precision of a ...
Recently, we developed a new method for measuring the thermodynamic stability of proteins. The method, termed Stability of Unpurified Proteins from R ...
Thermodynamic studies of pressu ...
and temperature at both low(19 bar) and high (318 bar) pressures using a homologous series of hydrophobic poly-l-phenylalanine (n = 2–7) as the model ...
Mathematical model of heat tran ...
We present a mathematical model that describes heat and mass transfer during solid-state fermentation (SSF) of Aspergillus oryzae in a well-mixed rot ...
Molecular thermodynamics and bi ...
Molecular thermodynamics finds application in biological systems in a number of areas, including separation and purification of biological molecules, ...
Selection of Highly Productive ...
In mammalian cell culture, the selection of high producers is a critical step in efficient recombinant protein production. Drug-resistance selection ...
Cell and process design for tar ...
This paper is a review of strategies to introduce protein into the liquid medium of Escherichia coli K-12 industrial production cells. The cell desi ...
A DNA vaccine against foot-and- ...
A plasmid DNA vaccine candidate (pCEIS) encoding two foot-and-mouth disease virus (FMDV) VP1 epitopes (amino acid residues 141–160 and 200–213) has b ...
Effects of acrylic acid on prep ...
Dextran hydrogels were obtained by radical copolymerization of methacrylated dextran (MA-dextran) with acrylic acid (AAc) using ammonium peroxydisulf ...
B-Casein Bilayer Adsorption at ...
Ellipsometric and surface pressure studies of â-casein adsorption layers at the water/air interface support the idea of a model that assumes the for ...
Selection of Highly Productive ...
In mammalian cell culture, the selection of high producers is a critical step in efficient recombinant protein production. Drug-resistance selection ...
Novel Escherichia coli Strain A ...
An important characteristic of promoters used in recombinant protein production in Escherichi coli is their inducibility in a simple and cost-effect ...
State of the art in the deliver ...
In photodynamic therapy, one of the problems limiting the use of many photosensitizers (PS) is the difficulty in preparing pharmaceutical formulation ...
High-performance tangeptial flo ...
High-performance tangential flow filtration (HPTFF) is a new technology for protein and nucleotide purification. Conventional ultrafiltration (UF) is ...
Metabolic Load of Recombinant P ...
The strong expression of recombinant proteins in bacteria affects the primary carbon and energy metabolism resulting in growth inhibition and acetat ...
Metabolic Adaptation of Escheri ...
The metabolic burden and the stress load resulting from temperature-induced production of human basic fibroblast growth factor is connected to an in ...
Limiting Factors in Escherichia ...
Fed-batch production of recombinant -galactosidase in E. coli was studied with respect to the specific growth rate at induction. The cultivations w ...
Characterization of enthalpic e ...
Protein adsorption can be either endothermic or exothermic depending upon the protein, the sorbent and process conditions. In the case of protein ads ...
Isothermal titration microcalor ...
The effect of (NH4)2SO4 concentrations on the interaction mechanism between myoglobin and two hydrophobic adsorbents, butyl- and octyl-Sepharose was ...
Preparative liquid chromatograp ...
The status of the theory and the main methods of implementation of preparative liquid chromatography are reviewed. On the theory front, the focus has ...
A theoretical analysis of the t ...
Although the denaturing of adsorbed proteins on biomaterials surfaces is believed to lead to adverse tissue reactions to implanted materials, very li ...
Interaction of Bovine Myelin Ba ...
Despite the essential role played by myelin basic protein (MBP) in stabilizing the multilamellar structure of the myelin membrane, attempts at decip ...