In vitro mutagenesis protocols

Site-directed mutagenesis using positive antibiotic selection / Richard N. Bohnsack -- In vitro site-directed mutagenesis using the unique restrictio ...

Recombinant gene expression pro ...

Recombinant gene expression protocols : overview of experimental strategies for the expression of recombinant genes / Rocky S. Tuan -- Designing expr ...

Protein purification protocols

General strategies / Shawn Doonan -- Preparation of extracts from animal tissues / Shawn Doonan -- Protein extraction from plant tissues / Peter R. ...

PCR cloning protocols : from mo ...

PCR sequencing protocols

Preparation and analysis of DNA sequencing gels -- Purification of PCR products from agarose gels for direct sequencing -- Enzymatic fluorescence an ...

Protein and peptide analysis by ...

Mass spectrometry in the analysis of peptides and proteins, past and present / Peter Roepstorff -- Mass spectrometry : ionization methods and instrum ...

Protein sequencing protocols

Protein sequencing protocols

Strategies for handling polypeptides on a microscale / Bryan John Smith, Paul Tempst -- SDS polyacrylamide gel electrophoresis for N-terminal protein ...

Glycoanalysis protocols

Characterization of protein glycosylation / Elizabeth F. Hounsell -- Analytical methods for the study of O-GlcNAc glycoproteins and glycopeptides / K ...

Sequence data analysis guideboo ...

GeneJockeyII : entering and editing sequences / Phil Taylor -- The genetic data environment : a user modifiable and expandable multiple sequence anal ...

Gene Targeting Protocols

PCR in bioanalysis

Application of nucleic acid amplification in clinical microbiology / Gorm Lisby -- Detection of rodent parvoviruses by PCR / David G. Besselsen -- T ...

Epitope mapping protocols

Choosing a method for epitope mapping / Glenn E. Morris -- Crystallographic studies of antigen-antibody interactions / Frederick A. Saul, Pedro M. Al ...

cDNA library protocols

cDNA library construction from small amounts of RNA using paramagnetic beads and PCR / Kris N. Lambert, Valerie M. Williamson -- Increasing the avera ...

Plant virology protocols

Ribozyme protocols

Ribozymes : an introduction / Helen A. James, Philip C. Turner -- Computer-aided calculation of the local folding potential of target RNA and its use ...

Combinatorial peptide library p ...

Synthesis of a one-bead one-compound combinatorial peptide library / Kit S. Lam, Michal Lebl -- Enzyme-linked calorimetric screening of a one-bead on ...

Neurotransmitter methods

Preparation of brain slices / Ting Wang, Ira S. Kass -- Preparation of organotypic hippocampal slice cultures using the membrane filter method / Pete ...

PRINS and in situ PCR protocols

Oligonucleotide PRINS DNA synthesis / John R. Gosden, Diane Lawson -- Chromosome-specific PRINS / Jean-Paul Charlieu, Franck Pellestor -- Bright-fie ...

Protein synthesis : methods and ...

Neuropeptide protocols

Preparation of neuropeptide-containing fractions from biological materials / J. Michael Conlon -- Purification of extracted peptides for structural a ...

Recombinant protein protocols : ...

Overview of experimental strategies on the detection and isolation of recombinant proteins and their applications / Rocky S. Tuan -- Reporter systems ...

Basic DNA and RNA protocols

The simultaneous isolation of RNA and DNA from tissues and cultured cells / Frank Merante, Sandeep Raha, Juta K. Reed, Gerald Proteau -- Restriction ...

Antibacterial peptide protocols

Origins and development of peptide antibiotic research : from extracts to abstracts to contracts / John K. Spitznagel -- HPLC methods for purificatio ...

Receptor signal transduction pr ...

Radioligand-binding methods for membrane preparations and intact cells / Mary Keen -- Site-directed mutagenesis and chimeric receptors in the study o ...