Biomembrane protocols

v. 1. Isolation and analysis -- v. 2. Architecture and function. [v. 1.] The identification of subcellular fractions from mammalian cells / John M. Graham -- The isolation of nuclei and nuclear membranes from rat liver / John M. Graham -- Isolation of mitochondria, mitochondrial membranes, lysosomes, peroxisomes, and golgi membranes from rat liver / John M. Graham -- Continuous-flow electrophoresis : application to the isolation of lysosomes and endosomes / John M. Graham -- Fractionation of a microsomal fraction from rat liver or cultured cells / John M. Graham -- Isolation of plasma membrane sheets and plasma membrane domains from rat liver / Laura Scott, Michael J. Schell, Ann L. Hubbard -- The analysis of receptor-mediated endocytosis by centrifugation in isoosmotic gradients / Terry C. Ford -- The isolation of clathrin-coated vesicles and purification of their protein components / Antony P. Jackson -- Isolation of membranes from tissue culture cells / John M. Graham -- The isolation of membranes from bacteria / Robert K. Poole -- Isolation and purification of functionally intact chloroplasts from leaf tissue and leaf tissue protoplasts / David G. Whitehouse, Anthony L. Moore -- Isolation and purification of functionally intact mitochondria from plant cells / Anthony L. Moore, Anne-Catherine Fricaud, Andrew J. Walters, David G. Whitehouse -- Isolation of cholinergic-specific synaptosomes by immunoadsorption / J. Paul Luzio, Peter J. Richardson -- Separation and analysis of phospholipids by thin layer chromatography / Ian J. Cartwright -- Analysis of phospholipids by high performance liquid chromatography / Hafez A. Ahmed -- Measurement of cholesterol in membranes / Hafez A. Ahmed -- Analysis of fatty acids by gas liquid chromatography / Ian J. Cartwright -- Chemical assays for proteins / David J. Winterbourne -- Separation and analysis of membrane proteins by SDS-polyacrylamide gel electrophoresis / Michael J. Dunn, Samantha J. Bradd -- Analysis of membrane proteins by Western blotting and enhanced chemiluminescence / Samantha J. Bradd, Michael J. Dunn -- Two-dimensional polyacrylamide gel electrophoresis of membrane proteins / Joe Corbett, Michael J. Dunn -- Isolation of a membrane-bound enzyme, 5'-nucleotidase / J. Paul Luzio, Elaine M. Bailyes -- The isolation of membrane proteoglycans / Malcolm Lyon -- Oligosaccharide mapping and sequence analysis of glycosaminoglycans / Jeremy E. Turnbull -- Analysis of sugar sequences in glycoproteins by glycosidase digestion and gel filtration / Anthony P. Corfield -- The extraction and analysis of glycosphingolipids / Norman A. Gregson -- [v. 2.] Crystallization of membrane proteins for X-ray analysis / Brian J. Sutton, Maninder K. Sohi -- Determination of cell-surface polarity by solid-phase lactoperoxidase lodination / William A. Muller -- Biochemical methods to determine cell-surface topography / William A. Muller -- Use of antipeptide antibodies for the isolation and study of membrane proteins / Stephen A. Baldwin -- The production of monoclonal antibodies to membrane proteins / Lynda J. Partridge -- Puriffication of a membrane protein (Ca²⁺/Mg²⁺-ATPase) and its reconstitution into lipid vesicles / J. Malcolm East -- Measurement of protein-protein interactions in reconstituted membrane vesicles using fluorescence spectroscopy / Anthony G. Lee -- Measurement of lipid-protein interactions in reconstituted membrane vesicles using fluorescence spectroscopy / Anthony G. Lee -- Determination of the transverse topography of membrane lipids using enzymes and covalent labels as probes / Joan A. Higgins -- Determination of the transverse topography of membrane phospholipids using phospholipid transfer proteins as tools / Joan A Higgins -- Prothrombinase complex as a tool to assess changes in membrane phospholipid asymmetry / Paul Comfurius, Edouard Bevers, Robert F. Zwaal -- Fluorescent glycerolipid probes : synthesis and use for examining intracellular lipid trafficking / Richard G. Sleight -- Synthesis and use of spin-labeled lipids for studies of the transmembrane movement of phospholipids / Pierre Fellmann, Alain Zachowski, Philippe F. Devaux -- Measurement of membrane fluidity and membrane fusion with fluorescent probes / Lindsay Bashford C. -- Extraction and assay of cyclic nucleotides / Guy St. Whitley -- Analysis of G-proteins regulating signal transduction pathways / Margaret M. Harnett -- Assay of protein kinases and protein phosphorylation / Margaret M. Harnett -- Analysis of cellular phosphoinositides and phosphoinositols by extraction and simple analytical procedures / Ian M. Bird -- Analysis of cellular phosphoinositides and phosphoinositols by high-performance liquid chromatography / Ian M. Bird -- Cytosolic free calcium measurements in single cells using calcium-sensitive fluorochromes / Mone Zaidi, A. S. Towhidul Alam, Christopher Box, Vijay Shankar, Peter J. Bevis, Christopher L. Huang, Michael Pazianas, Baljit S. Moonga -- Membrane permeabilization with bacterial toxins / C. Lindsay Bashford -- Measurement of ion fluxes and pH gradients across cell membranes / C. Lindsay Bashford -- Ligand binding and processing : the perfused liver / Carol A. Renfrew, Livia A. Casciola-Rosen, Ann L. Hubbard -- The binding of protein-ligands to cell-surface receptors / David A. Grant