PCR sequencing protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ralph Rapley
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 1996
- شابک / ISBN : 9780896033443
Description
Preparation and analysis of DNA sequencing gels -- Purification of PCR products from agarose gels for direct sequencing -- Enzymatic fluorescence and biotin labeling of primers for PCR sequencing -- Direct sequencing of double-stranded PCR products with the sequenase kit and [alpha-³⁵S] dATP -- Direct sequencing by thermal asymmetric PCR -- Rapid sequencing of cDNA clones : direct sequencing using sequential linear/asymmetric PCR -- Direct sequencing of PCR products using chemiluninescent detection -- Direct DNA sequencing of PCR products using magnetic beads -- Affinity capture and solid-phase sequencing of biotinylated PCR products -- Analysis of nucleotide sequence variations by solid-phase minisequencing -- Nonradioactive PCR sequencing using digoxigenin -- Silver Sequencing nonradioactive cycle sequencing of dsDNA -- Direct sequencing of PCR products with DNA-binding proteins -- PCR sequencing with the aid of detergents -- Direct sequencing with highly degenerate and inosine-containing primers -- Determination of unknown genomic sequences without cloning -- DNA sequencing by the chemical method -- Direct PCR sequencing with denaturants (formamide) -- Efficient PCR production of single-stranded DNA sequencing templates -- Preparation and direct automated cycle sequencing of PCR products -- Solid-phase automated sequencing of PCR-ampilified genomic DNA -- Cloning PCR products for sequencing in M13 vectors -- Sequencing PCR products cloned into M13 vectors -- Genomic ampilificatin with transcript sequencing (GAWTS) -- DNA rescue by the vectorette method -- Sequencing of (dA:dT) cloned mixed PCR products from microbial populations.
