G protein signaling : methods and protocols

Purification of recombinant g protein alpha subunits from escherichia coli / W.K. Greentree and M.E. Linder -- Purification of g protein subunits from sf9 insect cells using hexahistidine-tagged alpha and beta gamma subunits / T. Kozasa -- Expression and purification of soluble adenylyl cyclase from escherichia coli / J.A. Beeler and W.J. Tang -- Purification of phospholipase c beta and phospholipase c epsilon from sf9 cells / M. Ghosh ... [et al.] -- Assay for g protein-dependent activation of phospholipase c beta using purified protein components / M. Ghosh and A.V. Smrcka -- Assays of recombinant adenylyl cyclases expressed in sf9 cells / R. Taussig -- Measurement of g protein-coupled receptor-stimulated phospholipase d activity in intact cells / S.J. Walker and H.A. Brown -- Analysis of g protein-mediated activation of phospholipase c in cultured cells / B. Liu and D. Wu -- Intensive mutational analysis of g protein-coupled receptors in yeast / A. Celic ... [et al.] -- Green fluorescent protein-tagged beta-arrestin translocation as a measure of g protein-coupled receptor activation / S.S. Ferguson and M.G. Caron -- Detection of g protein-coupled receptors by immunofluorescence microscopy / P.M. Hinkle and J.A. Puskas -- Assay of g protein-coupled receptor activation of g proteins in native cell membranes using [35s]gtp gamma s binding / J.M. Bidlack and A.L. Parkhill -- Analysis of the coupling of g12/13 to g protein-coupled receptors using a luciferase reporter assay / B. Liu and D. Wu -- Selective inhibition of g protein-mediated pathways using rgs domains / T. Kozasa and R.D. Ye -- Ribozymes as tools for suppression of g protein gamma subunits / J.D. Robishaw, Z.P. Guo and Q. Wang -- In vivo adenoviral-mediated gene transfer of the beta arkct to study the role of g beta gamma in arterial restenosis / G. Iaccarino and W.J. Koch -- Analysis of rgs protein palmitoylation / L.S. Bernstein, M.E. Linder and J.R. Hepler -- Methods for measuring rgs protein phosphorylation by g protein-regulated kinases / S. Hollinger and J.R. Hepler -- The use of green fluorescent proteins to view association between phospholipase c beta and g protein subunits in cells / S. Scarlata and L. Dowal -- Cellular localization of gfp-tagged alpha subunits / T.R. Hynes, T.E. Hughes and C.H. Berlot.