Characterization of ex vivo healthy human axillary lymph nodes with high resolution 7 Tesla MRI
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : M. A. Korteweg & J. J. M. Zwanenburg & P. J. van Diest & M. A. A. J. van den Bosch & P. R. Luijten & R. van Hillegersberg &W. P. Th. M. Mali &W. B. Ve
- چاپ و سال / کشور: 2010
Description
Objective To characterize ex vivo healthy human axillary lymph nodes on 7 TeslaMRI and to correlate the findings with pathological analysis as a first step towards non-invasive staging of breast cancer patients in the future. Methods Four axillary lymph node dissection (ALND) specimens from 2 autopsy patients, who had no cancer, were examined on a clinical 7 Tesla MRI system. For morphological analysis a 3D T1-weighted fat-suppressed fast-field-echo [isotropic resolution 180 ىm] was acquired. For quantitative analyses 2D T1-, 3D T2-, T2*- and diffusion-weighted images were acquired. The ALNDs were mapped and stained for precise correlation of MRI to pathology. Nodes were sliced in 3 ىm sections, Haematoxylin & Eosin stained, and examined by an experienced pathologist. Results MRI detected all 45 nodes and 6 additional nodes that were not detected at pathological analysis. B-cell follicles, efferent- and afferent lymph vessels and blood vessels were identified. Mean T1, T2, T2*, ADC values (± standard deviation) were 944±113 ms, 32±2 ms, 16± 2 ms, 0.39±0.09·10.3 mm2/s, respectively. Conclusions 7 Tesla MRI of ex vivo human axillary lymph nodes correlated well with pathology. MRI detected all nodes present in the specimens and allowed visualization of fine structural detail. Pathology-correlated quantitative MRI data are presented.
Eur Radiol (2011) 21:310–317 DOI 10.1007/s00330-010-1915-3 Received: 22 March 2010 / Revised: 18 June 2010 / Accepted: 21 July 2010 / Published online: 9 August 2010
