Proximity ligation in situ assay for monitoring the global DNA methylation in cells`
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Eric Hervouet1,2, Philippe Hulin2,3, François M Vallette1,2 and Pierre-François Cartron1,2*
- چاپ و سال / کشور: 2011
Description
Background: DNA methylation has a central role in the epigenetic control of mammalian gene expression, and is required for X inactivation, genomics imprinting and silencing of retrotransposons and repetitive sequences. Thus, several technologies have been developed to measure the degree of DNA methylation. Results: We here present the development of the detection of protein-protein interactions via the adaptation of the proximity ligation in situ technology to evaluate the DNA methylation status in cells since the quantification of Dnmt1/PCNA interaction in cells reflects the degree of DNA methylation. Conclusion: This method being directly realizable on cells, it appears that it could suggest a wide range of applications in basic research and drug development. More particularly, this method is specially adapted for the investigations realized from a weak quantity of biologic materiel such as stem cells or primary cultured tumor cells for examples
Hervouet et al. BMC Biotechnology 2011, 11:31 http://www.biomedcentral.com/1472-6750/11/31
