A visible, targeted high-efficiency gene delivery and transfection strategy
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Qiao-ying Yuan (qiaoy99@163.com) Jing Huang (jingh99@126.com) Bao-Cheng Chu (chubc@u.washington.edu) Xing-Sheng Li (xingsh_li@163.com) Liang-Yi Si
- چاپ و سال / کشور: 2011
Description
Background: To enhance myocardial angiogenic gene expression, a novel gene delivery strategy was tested. Direct intramyocardial injection of an angiogenic gene with microbubbles and insonation were applied in a dog animal model. Dogs received one of the four different treatments in conjunction with either the enhanced green fluorescence protein (EGFP) gene or the hepatocyte growth factor (HGF) gene: gene with microbubbles (MB) and ultrasound (US); gene with US; gene with MB; or the gene alone. Results: Distribution of MB and the gene in the myocardium was visualized during the experiment. Compared with the EGFP gene group, an average 14.7-fold enhancement in gene expression was achieved in the EGFP+MB/US group (P < 0.01). Compared with the HGF gene group, an average 10.7-fold enhancement in gene expression was achieved in the HGF+MB/US group (P < 0.01). In addition, capillary density increased from 20.8 ± 3.4/mm2 in the HGF gene group to 146.7 ± 31.4/mm2 in HGF+MB/US group (P < 0.01). Conclusions: Thus, direct intramyocardial injection of an angiogenic gene in conjunction with microbubbles plus insonation synergistically enhances angiogenesis. This method offers an observable gene delivery procedure with enhanced expression efficiency of the delivered gene.
BMC Biotechnology 2011, 11:56 doi:10.1186/1472-6750-11-56 ISSN 1472-6750 Article type Research article Submission date 11 December 2010 Acceptance date 21 May 2011 Publication date 21 May 2011
