Improving RNA-Seq expression estimates by correcting for fragment bias
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Adam Roberts1, Cole Trapnell2,3, Julie Donaghey2, John L Rinn2,3 and Lior Pachter1,4
- چاپ و سال / کشور: 2011
Description
The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based approach. We find improvements in expression estimates as measured by correlation with independently performed qRT-PCR and show that correction of bias leads to improved replicability of results across libraries and sequencing technologies
Roberts et al. Genome Biology 2011, 12:R22
