Gene expression changes with differentiation of cord blood stem cells to respiratory epithelial cells: a preliminary observation
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Michael J Berger (berg0754@umn.edu) Sharon R Minnerath (minne002@umn.edu) Sheryl Adams (adams534@umn.edu) Barbara M Tigges (barbaratigges@hotmail.c
- چاپ و سال / کشور: 2011
Description
Introduction Owing to wide availability, low cost and avoidance of ethical concerns, umbilical cord blood (UCB) provides an attractive source of stem cells for investigational and therapeutic uses. With this study, we sought to characterize the gene expression changes as stem cells from UCB differentiate towards type II pneumocytes (ATII). Methods Control and experimental cells were cultured in maintenance (MSCGM) or differentiation (SAGM) medium, respectively, for 8 days. Total RNA was isolated from control and experimental groups for gene expression profiling and real-time (RT)-PCR. Results Analysis of only mixed cell lines (2) with parameters including a P value of 0.01 and an intergroup gap of 2.0 yielded a set of 373 differentially expressed genes. Prominently up-regulated genes include several genes associated with ATII cells and also lung cancers: ALDH3A1; VDR and CHKA. Several up-regulated genes have been shown to be integral or related to ATII functioning: SGK1; HSD17B11 and LEPR. Finally several up-regulated genes appear to play a role in lung cancers: FDXR and GP96. Down regulated genes appear to be associated with bone, muscle and central nervous system as well as other widespread tissues. Conclusions To the best of our knowledge, this accounting of the gene expression changes associated with the differentiation of a human UCB-derived stem cell towards an ATII cell represents the first such effort. Dissecting out which components of SAGM affect specific gene regulation events is warranted
ISSN 1757-6512 Article type Research Submission date 28 October 2010 Acceptance date 13 April 2011 Publication date 13 April 2011
