Defective Interfering Virus Protects Elderly Mice from Influenza
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Paul D Scott (p.d.scott@warwick.ac.uk) Bo Meng (b.meng@warwick.ac.uk) Anthony C Marriott (tony@southbank3.wanadoo.co.uk) Andrew J Easton (a.j.easto
- چاپ و سال / کشور: 2011
Description
Background. We have identified and characterised a defective-interfering (DI) influenza A virus particles containing a highly deleted segment 1 RNA that has broadspectrum antiviral activity. In young adult mice it exerts protection against several different subtypes of influenza A virus (defined here as homologous or genetically compatible protection) and against a paramyxovirus and an influenza B virus (heterologous or genetically unrelated protection). Homologous protection is mediated by replication competition between the deleted and full-length genomes, and heterologous protection occurs through stimulation of innate immunity, especially interferon type I. Methods. A single dose of the protective DI virus was administered intranasally to elderly mice at -7, -1 and +1 days prior to intranasal challenge with influenza A virus. Results. A single dose of the DI virus given 1 or 7 days protected elderly mice, reducing a severe, sometimes fatal disease to a subclinical or mild infection. In contrast, all members of control groups treated with inactivated DI virus before challenge became extremely ill and most died. Despite the subclinical/mild nature of their infection, protected mice developed solid immunity to a second infectious challenge. Conclusions. The defective interfering virus is effective in preventing severe influenza A in elderly mice and may offer a new approach to protection of the human population.
ISSN 1743-422X Article type Research Submission date 19 January 2011 Acceptance date 9 May 2011 Publication date 9 May 2011
